The pineapple variety N36 was amongst the four main varieties of pineapples planted in Malaysia [1]. This
variety is mainly used for canned product. The pineapple crown leaves are the burden of the canned pineapple
industry because the need of these pineapple crown for replanting is relatively small compared to the amount
of the refuse generated [2]. Hence, alternatives to its efficient utilization are necessary [3]. Fortunately, the
pineapple crown contains bromelain enzyme [4]. Bromelain is proteolytic enzymes or proteases [5]. It is an
aqueous extract of pineapple contains a complex mixture of proteases and non-protease components.
Bromelain is used in food processing for meat tenderisation