for 48 h. The cell suspension was then prepared and added to 10 mL of sterile distilled water and counted with a haemocytometer. The cell suspension was adjusted to 1 × 108 cells/mL (Nunes et al., 2001; Yu et al., 2007).
for 48 h. The cell suspension was then prepared and added to 10 mLof sterile distilled water and counted with a haemocytometer. Thecell suspension was adjusted to 1 × 108 cells/mL (Nunes et al., 2001;Yu et al., 2007).