Signs of chlorosis appeared first in interveinal areas, while midrib and veins remained green even in severely deficient leaves.
Concurrently, the results obtained indicate that changes observed in the Fe content and concentrations were accompanied by parallel changes in total Chl concentration, suggesting a close relationship between the two variables. These findings are consistent with a study by Jiménez et al. (2009), who found that the Fe concentration was lower in mesophyll leaf areas than in midrib and veins in a Prunus hybrid under controlled conditions, which could be associated with chlorophyll concentration and chlorosis symptoms.