2.1. Plant material, growth conditions, and salt treatment
Oryza sativa rice seeds of varying genotypes were obtained from
The Field Crops Research Institute, Giza, Egypt. These genotypes
were chosen from the germplasm collections based on their
reputation of salt tolerance in terms of agronomic performance.
The subset of the germplasm samples included the subspecies
O. sativa ssp. indica and O. sativa ssp. japonica.
Rice seeds were surface sterilized via immersion in a 5% sodium
hypochlorite solution for 30 min, and were then thoroughly rinsed
with distilled water. Seeds were subsequently soaked in tap water
for 24 h at 28 C. After germination, the seeds were transferred to a
nylon mesh floating on 20 L of tap water for two days. Water was
then replaced with half-strength Kimura B solution. Twenty-eightday
old seedlings (4e5 leaf stage) were transferred to either Kimura
B nutrient solution (control) or nutrient solution supplemented
with 50 mM NaCl (salinity) for two weeks. The solutions were
replaced every two days and the pH was adjusted to 5.0e5.5 each
day. Seedlings were grown in a growth chamber under the
following controlled environmental conditions: 70% relative humidity,
24 ± 2 C, and a 16 h photoperiod at a photosynthetic
photon flux density of 250e350 mmol m2 s1.
2.1. Plant material, growth conditions, and salt treatmentOryza sativa rice seeds of varying genotypes were obtained fromThe Field Crops Research Institute, Giza, Egypt. These genotypeswere chosen from the germplasm collections based on theirreputation of salt tolerance in terms of agronomic performance.The subset of the germplasm samples included the subspeciesO. sativa ssp. indica and O. sativa ssp. japonica.Rice seeds were surface sterilized via immersion in a 5% sodiumhypochlorite solution for 30 min, and were then thoroughly rinsedwith distilled water. Seeds were subsequently soaked in tap waterfor 24 h at 28 C. After germination, the seeds were transferred to anylon mesh floating on 20 L of tap water for two days. Water wasthen replaced with half-strength Kimura B solution. Twenty-eightdayold seedlings (4e5 leaf stage) were transferred to either KimuraB nutrient solution (control) or nutrient solution supplementedwith 50 mM NaCl (salinity) for two weeks. The solutions werereplaced every two days and the pH was adjusted to 5.0e5.5 eachday. Seedlings were grown in a growth chamber under thefollowing controlled environmental conditions: 70% relative humidity,24 ± 2 C, and a 16 h photoperiod at a photosyntheticphoton flux density of 250e350 mmol m2 s1.
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