In the experiment with residual glucose addition during part of the stationary phase (experiment 2), despite a
maximum vegetative cell concentration lower than in the other experiments, due to a lower glucose addition
during the exponential growth phase, a higher sporulation efficacy was reached (approximately 31%) leading to
a final spore concentration of 2.6 × 10
10
spores/mL. This higher efficacy indicates that for the sporulation
process both, nitrogen and glucose levels must be low to avoid cell lysis, although it is also necessary to supply
glucose to the culture permitting enough energy for the sporulation process to be completed.