In summary, an interface prepared via UV-hydrosilylation of 1,8-nonadiyne on Si(111) has been develop for the detection of DNA.
This sensor satisfy the basic criteria of being a robust and selective sensor, i.e., it stably immobilised DNA and resist the formation of insulating surface oxides during electrochemistry which changes the electronic properties of the interface.
Using BSA to prevent nonspecific adsorption of DNA, the recognition interface can successfully
detect complementary, non-complementary and single-base pair mismatched DNA.
Hence, we believe this is the first DNA modified surface suitable for the electrochemical detection of DNA hybridization on silicon electrodes.
In summary, an interface prepared via UV-hydrosilylation of 1,8-nonadiyne on Si(111) has been develop for the detection of DNA.This sensor satisfy the basic criteria of being a robust and selective sensor, i.e., it stably immobilised DNA and resist the formation of insulating surface oxides during electrochemistry which changes the electronic properties of the interface. Using BSA to prevent nonspecific adsorption of DNA, the recognition interface can successfullydetect complementary, non-complementary and single-base pair mismatched DNA. Hence, we believe this is the first DNA modified surface suitable for the electrochemical detection of DNA hybridization on silicon electrodes.
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