The rhizobacterial isolate RM-2 was characterized on the basis of sequencing of a partial 1270-bp fragment
of 16S rDNA amplicon and validated as Bacillus sp. In view of using this strain as bioinoculant for
sustainable agriculture in semi arid deserts, the plant growth promoting attributes were tested both in
laboratory and field conditions. The isolate was found to have many beneficial attributes such as P solubilization,
ACC deaminase activity, antifungal activity and ammonia and indole acetic acid production.
The isolate positively influenced the growth and nutrient uptake of cowpea plants. Seeds coated with
RM-2 showed a significant (P < 0.05) increase in seed germination, shoot and root length, fresh weight
and dry weight, and leaf area. It was also able to colonize the rhizosphere of plants. Yield parameters
such as number of pods and seeds and grain yield plant−1 were also enhanced significantly (P < 0.05) in
comparison to control in pot and field trials in natural semi arid conditions. RM-2 strain can be considered
as a good addition to consortium of beneficial microbes for growth promotion of cowpea plants.