Two-stage batch culture
In the two stage batch culture, cell dry weight of Ps. fluorescens S48 and
PHAs concentration were increased gradually during the second stage of
cultivation (production stage) to reach the maximum values after 60 hr in
productive medium containing either of 2 % corn oil or 1 % soybean oil (4.35,
2.96 gl-1
and 4.53, 3.44 gl-1
), respectively (Fig. 2). The highest values of PHAs
content was attained after 48 h (68.7 %) using corn oil and 78.2 % using soybean
oil, respectively. The corresponding figures of yield, productivity and conversion
coefficient were 39.9 %, 0.057 gl-1
h
-1
, 74.9 % and 45.7 %, 0.066 gl-1
h
-1 & 67 %
for corn oil and soybean oil, respectively.