the hydrolysate, and
ethanol concentrations were analyzed by using High Performance Liquid Chromatograph (HPLC). The
HPLC (Waters, USA) system was equiped with AMINEX HPX 87H column, and a guard column, an
automated sampler, a gradient pump, and a refractive index detector. The mobile phase was 5mM H2SO4
at flow rate of 0.6 mL min-1 and oven temperature was maintained at 65oC. Prior to HPLC injection, all
samples (derived from solids and hydrolysate) were neutrilized with calcium carbonate, shake for 2 min,
and filtered through 0.2 m syringe filters.
the hydrolysate, andethanol concentrations were analyzed by using High Performance Liquid Chromatograph (HPLC). TheHPLC (Waters, USA) system was equiped with AMINEX HPX 87H column, and a guard column, anautomated sampler, a gradient pump, and a refractive index detector. The mobile phase was 5mM H2SO4at flow rate of 0.6 mL min-1 and oven temperature was maintained at 65oC. Prior to HPLC injection, allsamples (derived from solids and hydrolysate) were neutrilized with calcium carbonate, shake for 2 min,and filtered through 0.2 m syringe filters.
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