denaturation for 2 min at 92 oC, amplification for 10 cycles consisting of 10 sec at 92 oC, 30 sec at 60 oC and 2 min at 68 oC, continued with amplification for 20 cycles consisting of 15 sec at 92 oC, 30 sec at 60 oC and 2 min at 68 oC, with time prolongation of 20 sec for each cycle and ended with an extension at 68 oCtemperature for 7 min (Iskandriati et al. 2010).