The fungi were identified following sporulation.
Single spore subcultures were obtained for
each Colletotrichum isolate using the
procedure described by Goh (1999). When
direct examination showed that the fungus was
sporulating on the berry, spore masses were
picked off with a sterilized wire loop and
streaked on the surface of water agar. After
incubation overnight (28-30°C), single germinated
spores were picked up with a sterilized
needle and transferred to PDA. Pure cultures
were stored in sterilized water in Eppendorf
tubes at 6°C (Abang, 2003). Cultures are
deposited in the culture collection of Mae Fah
Luang University, BIOTEC Culture Collection
(BCC), and CBS.