These calli prevented the growth of shoots, leading to necrosis. Considering all the media comprehensively, the best medium for shoot multiplication of each cultivar was MS medium supplemented with BA 2.2 μM, NAA 0.054 μM and GA3 0.4 μM, on which shoot proliferation (≥3.0) and average height of shoot clumps (>1.3) was greater (Tab. 7).