Soil samples were collected from different localities at El-
Madina El-Monawara city (Qurban El Nazel – Eastern Hora
– Ohod mountain) Kingdom Saudi Arabia, and were kept at
25 C. Human saliva samples were taken from healthy persons.
One gram of soil, 1 ml of saliva and 1 ml of mixture (1 g
soil+ 1 ml of saliva) were inoculated to the nutrient Browth
medium for bacterial growth and Sabourand Dextrose liquid
medium for fungal growth, incubated for 10 days. Thereafter,
the culture media were filtered using sterilized bacterial filters
(NALGENE 0.45 Mm) produced by Nalge Nunc International.
Isolation of different bacterial and fungal isolates was
carried out from the mixture and purified. The bacterial isolates
were identified according to Bergey’s manual of systematic
bacteriology (Krieg and Holt, 1984; Sneath et al., 1986).
The fungal isolates were identified in Botany Department, Faculty
of Science, Cairo University, Egypt.