In the experiment performed, effect of different pH values i.e., 5.4, 6.4 and 7.4 were used to study germ tube formation in C. albicans keeping the temperature con-stant at 37°C (Figure 3). The peak germ tube formation was seen at pH 7.4 and about 40% cells form germ tube after 4.5 hr of culture inoculation (Table 3). It was also reported that the extracellular pH is one of the environ-mental factors that modified the physiology of the cell