Newly emerged worker honey bees were divided in groups and individually treated as later described. Workers were kept into plastic cups (cages) and maintained at 30±1 °C with a 70 ± 10% of RH in the dark. For all experiments, the caged bees were fed per os, using micropipette, with specific treatment solution and all bees in the cages were fed with sucrose water solution (1:1) ad libitum.