Five agar plugs
(5-mm-diameter) containing actively growing mycelium of
P. ramorum isolate WSDA-1772 were placed in 20 ml of 20% sterile,
clarified V8 broth in sterile Petri plates (100-mm-diameter) and
stored at 20 C in the dark. After 4 weeks, the mycelia and chlamydospores
were transferred to a blender cup containing 50 ml of
sterile water and blended for 20 s