proportion of methanol:water (8:2) in acetone varied from 10 to 90%, and the solid (g)–liquid (mL) ratio from 1:1 to 1:9 (m/v); extraction time and number of extractions were also investigated (from 5 to 25 min and from 5 to 25 extractions, respectively). After extraction, the extracts were stored at −36 °C until analysis.