In this study was used congo red method to detect cellulose hydrolysis because it was
very convenient to read results quickly and dye durability. (Theather and Wood, 1982) Separated by
a popular culture based on CMC agar and then stained with congo red also use other substances
instead (Lamb and Loy, 2005). It has developed methods by Kasana et al., (2008) used gram's
iodine staining instead congo red. Samples were isolated microorganisms that can produce
cellulase to find amount and type of microorganisms of different samples in each area. Prasertsan
(1992) determine the ability to produce cellulase by congo red method an initial inspection, which
must have been the operation of the enzymes by microorganisms in the next experiment.