The overall establishment of biosurfactants is well known to be impeded by a lack of availability of economic and versatile products. Currently there is only a very limited offer of commercially available biosurfactants, e.g., surfactin, sophorolipids and rhamnolipids. A variety of new biosurfactants respectively producing strains are the key issue in overcoming the economic obstacles of the production of biosurfactants. Therefore, increased efforts in the discovery of new biosurfactant producing microbes must be made by applying a broad range of different screening methods, which is the focus of this review.