The flowering aerial parts of Artemisia annua were collected at
the end of October 2011 in Sesto Fiorentino, Tuscany, Italy. The
plant was identified and the voucher specimen is deposited at the
Phytolab, Department of Chemistry, under the authentication
number AA 10/2011. The fresh plant material (1 kg) was coarsely cut
and hydro distilled in a Clevenger-like apparatus for 2 h. The oils
were dried over anhydrous Na2SO4, frozen, and stored in sealed
vials at 4 C before GC analysis. The oil was conserved at ?22 C for
the GCMS and microbiological assays.