As a result of the degradation and destruction of natural habitats, many species have
become threatened in situ and need conservation.One of the main tasks of Botanical
Gardens is local fl ora conservation, including the elaboration of ex situ conservation
measures. In accordance to the ex situ conservation strategy, it is not desirable to propagate plants from small and genetically restricted samples, because depletion of genetic variability may reduce the ability to adapt in habitats. Therefore, the material obtained from natural populations must represent a wide genetic base. Tissue culture is used for conservation of biological diversity for multiplying of endangered species that have extremely small populations, for species with reproductive problems and for recovery and reintroduction.