Biological filters were inoculated after 24 h taking into consideration the entire system water volume (VEf), with aerobic bacteria lyophilized (AZOO – NitriPro, Nitrosomona and Nitrobacter) at 3 g of bacteria per 250 L of water.
48 h later, feed was supplied again to the ponds (20 g each), and after 24 h a second bacterial inoculation was performed at 1 g per 250 L of water.