3.5. SSF process for ethanol productionThe influence of the pretreatment type (LHW, DSA, E&LHW, and E&DSA) and the substrate particle size (original size, 0.51e7.00 mm, and small size, <0.36 mm) on ethanol production was assessed by asimultaneous saccharification and fermentation of the pretreated solids. In general experimental data showed that ethanol concentration increased quickly during the first 24 h of SSF, and that after48 h slight increases of this parameter were observed (Fig. 5). The final concentration of bio-fuel was clearly influenced by the pretreatment type and the particle size. As expected, fermentationscarried out with the small size particles (SSP) achieved higher yields
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