After shaking, suitable dilutions were made in the same solution and were used to inoculate solid media of growth composed by 5 g L-1 carboxymethylcellulose (CMC) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany), 1 g L-1 (NH4)NO3, 1 g L-1 yeast extract, 50 ml L-1 standard salt solution, 1 ml L-1 trace elements solution, 0.02% Remazol Brilliant Blue R [19], 10 g L-1 bacteriological agar, at pH 7.0.