estimated ,
Saishi n analytical RP HPLC , integrated with Chemstation software, C-
Spherisorb, pore
size 5 μ; VWD (variable wavelength detector). All solvents were filtered
through Millipore filter disk. 20 μl of the pool B
injected to HPLC column. Isocratic mobile phase of
acetonitrile dichloromethane methanol
HPLC grade solvents were procured from Merck Mumbai. The flow
rate was 1.0 ml/min and absorbance was measured at 450 nm.