Although a full mechanistic understanding of probiotics stability in biopolymer matrices during storage is not available, it appears that factors such as steric hindrance of solutes and the matrix translational diffusion of oxygen (both associated with the T − Tg difference), the presence of nutrients and free radical scavenging agents as well as the interaction via hydrogen bonding with the polar head groups of membranes phospholipids can be possible explanations for the stability of probiotics in prebiotic films