The study was performed on male adults Wistar rats (90 days old) in accordance with the guidelines of both the
Organisation for Economic Cooperation and Development (OECD) and the Food and Drug Administration
(FDA). At the beginning of the test, the weight of each rat was chosen within a range of ±20% of the average
weight of all the animals used in this study with respect to the principles governing research on animals as defined
by the OECD (2000) [7] and the Canadian Council on Animal Care (CCAC) [8]. Then, the animals were
grouped and placed into scientific cages located in the animals room, which was maintained at a temperature of
22˚C to 24˚C and a humidity between 60% and 70%, and where the animals had free access to water and food
(standard food) The rats were maintained under these conditions for five days in order to acclimatize with the
environment.