2.6.4. HPLC analysis of anthocyanins Anthocyanins were determined using a Dionex (USA) HPLC system equipped with a model Ultimate 3000 diode array detector, a LPG-3400A quaternary pump, a EWPS-3000SI auto sampler and a TCC-3000SD thermo stated column compartment, controlled by Chromeleon v.6.8 software.