The reaction contained 50 ng template DNA, 1X PCR buffer, 1.5 Mm MgCl2, 0.2 mM dNTP, 250 nM each of forward and reverse primers and 0.25U Taq polymerase in a total volume of 10 ll. PCR amplification was carried out in an M&J Thermal cycler.
The reaction contained 50 ng template DNA, 1X PCR buffer, 1.5 Mm MgCl2, 0.2 mM dNTP, 250 nM each of forward and reverse primers and 0.25U Taq polymerase in a total volume of 10 ll. PCR amplification was carried out in an M&J Thermal cycler.