A volume of 0.3 mL of LWE with or without each additive and 109 CFU/mL of the bacterial suspensionwas placed in the PEF treatment chamber, as previously described (Monfort et al., 2010b). Microorganisms were treated in a tempered batch parallel-electrode treatment chamber (15.0 ± 0.1 °C) with a distance between electrodes of 0.4 cm and an area of 0.79 cm2.