Cyanobacteria
The
dominant algal species in the lake were the cyanobacteria,
2.2.2.
Anabaena
circinalis and Microcystis cf botrys, with the latter being
more
abundant than the former in summer 2012 (Lui, S., pers.
comm.
March, 2012). Scenedesmus sp. also occasionally occurs in
the
lake. Cultures of these species were not available for this study.
Instead,
unialgal strains of the cyanobacterium, A. variabilis and the
green
alga, S. quadricauda were used from long-term cultures held
in
the Algal Physiology Laboratory at Monash University. These cul-
tures
had originally been isolated from water samples collected on
campus
and are retained as strains Avar1 and Scenq1, respectively.
The
target organisms were cultured separately in 250 mL sterilised
Erlenmeyer flasks with 100 mL BG-11 (A. variabilis) and MLA
(S.
quadricauda) medium modified after Mohamed and Al Shehri
(2010)
and Bolch and Blackburn (1996), respectively. Flasks were
incubated
at 25
◦
C, a photon flux of 65 mol photons PAR m
and a 14:10 light:dark photoperiod.
−2
s
−1
CyanobacteriaThe dominant algal species in the lake were the cyanobacteria,2.2.2. Anabaena circinalis and Microcystis cf botrys, with the latter beingmore abundant than the former in summer 2012 (Lui, S., pers.comm. March, 2012). Scenedesmus sp. also occasionally occurs inthe lake. Cultures of these species were not available for this study.Instead, unialgal strains of the cyanobacterium, A. variabilis and thegreen alga, S. quadricauda were used from long-term cultures heldin the Algal Physiology Laboratory at Monash University. These cul-tures had originally been isolated from water samples collected oncampus and are retained as strains Avar1 and Scenq1, respectively.The target organisms were cultured separately in 250 mL sterilisedErlenmeyer flasks with 100 mL BG-11 (A. variabilis) and MLA(S. quadricauda) medium modified after Mohamed and Al Shehri(2010) and Bolch and Blackburn (1996), respectively. Flasks wereincubated at 25◦C, a photon flux of 65 mol photons PAR mand a 14:10 light:dark photoperiod.−2s−1
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