Drop collapse test
Qualitative drop collapse test was performed according to the
protocol of Youssef et al. (2004). 2 mL of diesel oil was added to the
96 well microtitre plates. The plates were equilibrated for 1 h at
37 C and 5 mL of culture broth supernatant was added to the
surface of the oil. The shape of drop on oil surface was observed
after 1 min. The culture supernatant that make the oil drop
collapsed was indicated as positive result and that drops remain
beaded were scored as negative. Distilled water was used as
negative control.
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