The tube was mix-vortexed, centrifuged at 16,000 g for 10 min; 100 lL of the supernatant solution were diluted with 100 lL 0.01 M ammonium formate, pH 3.5 (Sigma–Aldrich).
The tube was mix-vortexed, centrifuged at 16,000 g for 10 min;100 lL of the supernatant solution were diluted with 100 lL 0.01 M ammoniumformate, pH 3.5 (Sigma–Aldrich).