with highly sensitive methods such as ddPCR or assimilated
should be able to provide answers to these fundamental questions
(Laurent-Puig et al., 2015).
In conclusion, the data presented here suggest that it is
feasible to detect KRAS mutations in CTCs from blood samples
of colorectal cancer patients that are predictive for those present
in the tumor. The minimal invasive nature of this procedure
in combination with the high sensitivity of ddPCR might
provide in the future an opportunity to monitor patients
throughout the course of disease on multiple levels including
early detection, prognosis, treatment and relapse as well as to
provide improved mechanistic insight with respect to tumor
invasion and metastasis. Although the current study was
focused on KRAS mutations, this strategy should also allow
us to monitor other clinically relevant mutations, or combinations
of mutations, for improved patient care.