ight intensity was measured on top and at the bottom of the water column over a representative sampling day, by inserting a 4π light meter probe (Walz Universal Light Meter). See Fig. 1 for light distribution. Cells were acclimated to these conditions for approximately 5 generations (5 day:night cycles), diluted into fresh medium and grown for 2 more days before data collection. Time series data were collected during one diurnal cycle starting at dawn. Samples were harvested between 0 h and 0.5 h after dawn (dawn), between 3 h and 3.5 h after dawn (mid-morning), between 6 h and 6.5 h after dawn (mid-day), between 9 h and 9.5 h after dawn (mid-afternoon) and between 12 h and 12.5 h after dawn (dusk). 2nd dawn samples were collected 24 h after the first harvest.
Fig. 1