examined via FDA live cell staining under a florescence microscopeon the first and seventh day after seeding (Fig. 7B). The cells within the scaffolds began to spread, proliferate and infiltrate in the 3D structure after cell attachment over time. Yet,the images of infiltration were not captured due to the inability of the light microscope to focus on the Z-axis. Especially FCA/COS1 indicated the highest colour intensity in seventh day culture. Therefore, biological examinations of the scaffolds indicate that COS1 is the best molecular size to induce fibroblast cell proliferation after cross-linking with FCA.