4. Conclusions
Structural space for acceptance of substrates of different shapes in lipases from the groups of RmL, CaLA, CaLB and Cutinase has been explored. The RmL group allows large alcohol part substrate, but more limited/narrow acyl binding cleft on the acid part. CaLA group exhibits strong restriction on the acid part having a narrow tunnel to accommodate the acyl group from the acid part of the substrate and a much wider space on the alcohol part allowing highly branched and large groups on the alcohol part. The CaLB group shows more space on the acid part and less on the alcohol part and with restriction on the branching at CA carbon. Cutinase group has a reasonable open space on both the alcohol and the acid part of the substrate. This structural understanding was tested with the 8 substrates giving an overall result pointing in the same direction as the structural understanding.