The membrane was dried at 50oC under a dry wind blower for 10 - 15 min and blocked with PBS containing 2% ovalbumin and 1.5% sucrose. The membrane was dried at 50oC under a dry
wind blower for 10-15 min and washed twice with 0.01 M, sodium phosphate buffer, pH7.2 before drying it for 10 - 15 min at 50oC. A polyethylene plastic sheet (26 × 8 cm) of 0.2 mm thickness was coated with acrylic adhesive on one side and the 2.5 cm wide membrane was placed centrally at a spacing of 1.5 cm from the top and 4cm from the bottom end of the sheet. The conjugate coated glass-fiber pad was placed on the lower end of the membrane, so as to overlap 2mm on it. A filter pad was placed to overlap 2 mm on the lower end of the conjugate release pad to act as sample pad and another pad