2.7. Microbiological analysis
Raw sample (10 g) was placed in sterile plastic bags (Sterilin, Stone,
Staffordshire, UK) with 90 mL of peptone water. After 1 min in a stomacher
blender (Colworth 400, Seward, London, UK), different dilutions
were made up to determine the different microorganism counts. Aerobic
mesophilic bacteria were determined on 3M Petrifilm™ plates for
total counts, incubated at 35 °C for 48 h. Enterobacteria were determined
on 3M Petrifilm™ plates for enterobacteria incubated at 37 °C
for 24 h, Escherichia coli were determined on 3M Petrifilm™ plates for
E. coli incubated at 37 °C for 24 h and molds and yeasts were determined
on Rose Bengal plates with chloramphenicol incubated at 28 °C for
5 days. All results are reported as log10 colony forming per gram (CFU).
2.7. Microbiological analysisRaw sample (10 g) was placed in sterile plastic bags (Sterilin, Stone,Staffordshire, UK) with 90 mL of peptone water. After 1 min in a stomacherblender (Colworth 400, Seward, London, UK), different dilutionswere made up to determine the different microorganism counts. Aerobicmesophilic bacteria were determined on 3M Petrifilm™ plates fortotal counts, incubated at 35 °C for 48 h. Enterobacteria were determinedon 3M Petrifilm™ plates for enterobacteria incubated at 37 °Cfor 24 h, Escherichia coli were determined on 3M Petrifilm™ plates forE. coli incubated at 37 °C for 24 h and molds and yeasts were determinedon Rose Bengal plates with chloramphenicol incubated at 28 °C for5 days. All results are reported as log10 colony forming per gram (CFU).
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