2.3. Preparation of the inoculum
With the use of mixed inocula, the percentages of the proportion
Cicy-Ki/Saccharomyces were adjusted to 0/100, 25/75, 50/50,
75/25 and 100/0 of each strain, maintaining the same initial concentration
of 3 107 cell mL1 in every case. The inoculum volume
was 200 mL, and the medium was prepared in the same way as the
substrates of the corresponding fermentations. The inocula were
incubated at 35 ± 2 C in darkness and at 200 rpm for 24 h.