Analyses of recombinant OGNNV VLPs. (A) Electron microscopy of negatively stained OGNNV and (B) VLPs (Bar¼20 nm). (C) SDS-PAGE analysis and Western Blot analysis of
purified VLPs. Lane 1, BSA (1.0mg); Lane 2, Coat protein (1.0mg); M 1, SDS-PAGE Marker; M 2, Western Blot Marker. (D) Three-dimensional model of capsid protein of nervous
necrosis virus in orange-spotted grouper. (For interpretation of the references to colour in thisfigure legend, the reader is referred to the web version of this article.)