2.2. Extraction of carotenoids
Cephalothorax, cuticles, pleopods, and tails from shrimp (L.
vannamei) were homogenized to a particle size ofz5 mm. Aliquots
of the homogenate (10 g) were mixed with ethyl acetate (50 mL)
and stirred at room temperature in darkness for 30 min; after
extraction the sample was filtered through Whatman No. 1 filter