Fig. 4. Fermentation profile of pET21-60EGP transformed E. coli in defined media. A Batch culture without oxygen control. B Batch culture with oxygen control. C Fed batchculture with oxygen control. Square- Dissolved oxygen, Triangle- Glucose level, Inverted triangle- Acetate production, Circle- Biomass production. All experiments wereduplicated or triplicated. Roman numerals above graphics correspond to: I- Pre-induction, II-V- After induction with IPTG, III- first glucose feed, IV- second glucose feed, V-third glucose feed. Variations in each time point and the general trend were less than 5% within each replicated set.