To test whether LPS16 can adhere to gastric epithelial cells,
PKH2-labeled LPS16 was incubated with gastric epithelial
cells AGS and MKN45, and adherence ability was determined
by flow cytometry. LPS16 [multiplicity of infection
(MOI) Z 100] showed significant adherence to two cell lines
when compared to epithelial cells only (Fig. 3, ManneWhitney
U test, p < 0.05). However, LPS16 (MOI Z 100)
showed less adhesion ability than H. pylori J99 and 26695
(MOI Z 10 and MOI Z 20) on the two cell lines (Fig. 3,ManneWhitney U test, p < 0.05).