These plates were labeled and 100 µl of each plant extracts (at concentration of 50,100 mg/ml) was added aseptically into the well.
Then the plates were incubated for 24 h at 37ºC during which the activity was evidenced by the presence of zone of inhibition surrounding the well.
Each test was repeated three times and the antibacterial activity was expressed as the mean of diameter of the inhibition zones (mm) produced by the plant extracts when compared to the controls.
McFarland. Ten µl of each bacterial suspension was inoculated in peripheral lines in distance of 1.5 cm from central line by tooth pick. Triplicate plates were incubated at 28°C for 7 days in static condition. Antifungal activity was assessed by comparing the zone of fungal growth inhibition in fungi co-cultured with bacteria as tests, in comparison with control plates which were inoculated only with corresponding fungi.