2.3. Experimental design
The Design of experiments and subsequent analysis of results
were performed by Design expert 8.0 software (State-ease Inc.,
U.S.A.). Central composite rotatable design (CCRD) was employed in
the present study (Abreu, Beirão-da-Costa, Gonçalves, Beirão-da-
Costa, & Moldão-Martins, 2003). The variables optimized were citric
acid concentration (X1), irradiation dose (X2) and storage time
(X3). The independent variables were coded at five levels (a, 1,
0, þ1 and þa). Coded and actual values are shown in Table 1.
Complete design consisted of 20 experimental points including six
replications of the center points (all variables coded as zero) (Table 2).
2.3. Experimental designThe Design of experiments and subsequent analysis of resultswere performed by Design expert 8.0 software (State-ease Inc.,U.S.A.). Central composite rotatable design (CCRD) was employed inthe present study (Abreu, Beirão-da-Costa, Gonçalves, Beirão-da-Costa, & Moldão-Martins, 2003). The variables optimized were citricacid concentration (X1), irradiation dose (X2) and storage time(X3). The independent variables were coded at five levels (a, 1,0, þ1 and þa). Coded and actual values are shown in Table 1.Complete design consisted of 20 experimental points including sixreplications of the center points (all variables coded as zero) (Table 2).
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