Shoots of 2.5-cm length were then transferred to
one of four rooting media: (6) ½MS + 0.1 mg L-1 IBA;
(7) ½ MS+ 0.25 mg L-1 IBA; (8) ½ MS+ 0.5 mg L-1
IBA; or (9) ½ MS+ 1.0 mg L-1 IBA, each containing
20 g L-1 sugar and 6.0 g L-1 carrageenan, pH 5.8. There
were 30 jars of each medium and 3 replication for the
same experiment. The shoots were maintained under
15–25 μmol m-2 s-1 irradiance (12 h d-1) with a room
temperature of 26 2°C. Rooting percentage, root
number, root length and plantlet height were recorded
at the end of 60 days in rooting medium. Plantlets were
then transplanted into rectangle plastic baskets
containing red core soil (natural local soil) in
greenhouse.