For efflux experiments with individual fish, a single inanga was
placed in a separate container filled with 250 mL of experimen
hour for the next three hours, 24 h after initial handling)tal water (composition as above). Seven replicate fish were used
(n = 7, mass ranging from 0.7 to 1.4 g) and triplicate 5-mL water
samples taken attime zero, and every hour for the nextthree hours,
in order to determine Na+ concentrations. Fish were left overnight
(24 h) in exposure chambers to acclimate, after which the water