The replacement for traditional cultivation techniques of straw-berry fruit with soilless systems has steadily grown over the pastdecades in the region of Huelva (Spain) and elsewhere. Soillesscultivation of strawberry fruit is thought to have numerous envi-ronmental advantages over traditional systems, since water andnutrients are used in a more sustainable manner and the vulnera-ble soil reserves (Hernanz et al., 2007, 2008) are not degraded. Inthis context, it is well recognised that certain pre-harvest practicesor conditions may affect the taste- and health-related compositionof strawberry fruit as well as alter plant productivity. Nonetheless,the present study has shown that despite some minor differences in fruit yield, a number of different substrates with different physicochemical properties may be employed during soilless cultivationof strawberry fruit without affecting the final fruit quality or com-position. As reported in other studies