Effect of chitosan on protein leakage and lipid
peroxidation
Leakage loss of protein from mycelia into the culture medium
was substantial when S. sclerotiorum was cultured in PDB
containing 1% chitosan (pH 5.0). The protein concentration
in the medium rapidly increased during the first 24 h and then
continued to increase over the measurement period (Fig. 3).
In contrast, the level of protein in PDB medium without chitosan
remained low during the entire measurement period.
The pattern of lipid peroxidation when S. sclerotiorum was
cultured in PDB with and without 1% chitosan (pH 5.0) was